KMID : 0894520190230020111
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Development & Reproduction 2019 Volume.23 No. 2 p.111 ~ p.117
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Prostaglandin affects in vitro ovulation and 17¥á, 20¥â-Dihydroxy- 4-pregnen-3-one production in longchin goby, Chasmichthys dolichognathus oocytes
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Baek Hea-Ja
Lee Da-Som
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Abstract
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This study focused on the association of prostaglandins and a progestin, 17¥á, 20¥â-dihydroxy-4-pregnen-3-one (17¥á20¥âP) during the ovulation process in longchin goby, Chasmichthys dolichognathus. We performed several in vitro experiments using 850?920 ¥ìm diameter oocytes which were at the migratory nucleus stage. With the 890?920 ¥ìm diameter oocytes, no significant difference in ovulation was observed in any of the prostaglandins (PGE1, PGE2, and PGF2¥á) treated groups although PGE2 and PGF2¥á at concentrations of 50 ng/mL increased ovulation slightly compared with controls; however, 17¥á20¥âP production was stimulated with PGE1 alone at low concentrations (5 ng/mL). In 850 ¥ìm diameter oocytes, PGF2¥á at concentrations of 50 and 500 ng/ml resulted in a significant increase in ovulation. 17¥á20¥âP (50 ng/ml) alone had no observable effect on ovulation, but in the combined of PGF2¥á 50 or 500 ng/ml it caused the greatest effect on ovulation. The sensitivity of oocytes to the induction of ovulation varies between 850 and 890?920 ¥ìm, it appeared to vary depending on the migration status of nucleus. These results suggest that PGF2¥á (or combined of 17¥á20¥âP) was more potent in inducing ovulation of the longchin goby.
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KEYWORD
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Chasmichthys dolichognathus, Ovulation, Prostaglandins, 17¥á, 20¥â-dihydroxy-4-pregnen-3-one
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